Production and properties of dextransucrase by free and immobilized cells of

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Six bacterial strains were tested for the production of dextransucrase. The highest dextransucrase activities were produced by local strain after 24 h in static culture conditions. Dextransucrase was induced using sucrose (10 g%) as a carbon source and corn steep liquor (3.5 ml%) as a nitrogen source. Fe favoured enzyme production. Cells of were immobilized using three supports, calcium alginate, agar and κ-carrageenan. Calcium alginate beads were able to produce dextransucrase for four runs with 100% activity. The optimum temperature for enzyme reaction ranged from 30 to 65πC at pH 5.4. In the absence of substrate, the enzyme retained 100% activity for 30 min at 40πC and 69% activity for 10 min at 60πC.

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