Effect of five plant extracts on adhesion of  onto human buccal epithelial cells

Pavithra, Jain Amrath; Srinikethan, G; Shubhada, C; Pradeep, K; Gopala, Mugeraya; Kulkarni, Raghavendra; Praveenchandra, K R

doi:10.4103/1687-4315.147089

Effect of five plant extracts on adhesion of onto human buccal epithelial cells

Authors

Abstract

Background
There is increased prevalence of both fluconazole-resistant and non-albicans spp. isolated from oral candidiasis (OC) lesions. OC is the most common oral lesion, encountered in HIV infection. On the basis of this, the antiadherence approach to treat or prevent oropharyngeal candidiasis was studied using plant extracts.
Aim
The present study aimed to perform preliminary screening of five plant extracts, namely, aloe vera, Singapore cherries, tea tree, neem, and lemon grass, for their effect on adhesion of to human buccal epithelial cells (HBEC) in an in-vitro condition.
Materials and methods
A set of 5 and 10 g of plant material, leading to a final concentration of 10 and 20%, respectively, was used. Both aqueous and ethanol extracts were tested. Both and HBEC were treated with plant extracts under different in-vitro conditions.
Statistical analysis
Statistical analysis was carried out by two-way analysis of variance using IBM SPSS-version 20.
Results
Both aqueous and ethanol extracts of neem. followed by lemon grass were found to consistently inhibit adhesion, which was statistically significant.
Conclusion
This preliminary work has shown a trend that different plant extracts could efficiently inhibit the adherence of to HBEC and can be explored for an antiadherence therapeutic approach. Development of antiadherent agents using plant extracts seems to be a promising approach in the treatment of OC.

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