Estimation of total phenolic, tannins, and flavonoid contents and antioxidant activity of  heart wood extracts

Jain, Sourabh; Jain, Aakanchha; Jain, Sanjay; Malviya, Neelesh; Jain, Vikas; Kumar, Dharmendar

doi:10.4103/1687-4315.154690

Estimation of total phenolic, tannins, and flavonoid contents and antioxidant activity of heart wood extracts

Authors

Abstract

Purpose
The present study was investigated to determine antioxidant activity and total phenolic, total flavonoids, and total tannins contents of extracts of heart wood.
Materials and methods
Antioxidant activity of aqueous and alcoholic extract of heart wood was evaluated against 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picryl hydrazyl (DPPH), and hydroxyl radical-scavenging activity models. Total flavonoids, tannins, and phenolic content of were also determined.
Results and conclusion
Among both extracts, aqueous extract showed the highest total phenolic contents (23.97 μg/g of gallic acid equivalent/g of extract). In DPPH, superoxide anion, and ABTS scavenging test, the IC (μg/ml) value of aqueous and alcoholic extract was 61.89, 75.79, 87.76, 121.55, 115.29, and 122.42, respectively. Hence, the above evidences suggest that heart wood is a potential source of natural antioxidant and can be used to prevent diseases associated with free radicals.

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