The composition of the lipoidal matter of the seeds of  (DC.) Leenh

Said, Ataa A.; Aboutabl, Elsayed A.; Hussein, Ahmed A.; Abdel Raoof, Gehan F.

doi:10.4103/1687-4315.154725

The composition of the lipoidal matter of the seeds of (DC.) Leenh

Authors

Abstract

Background and objectives
Nothing dealing with the chemistry of the seeds of (DC.) Leenh. could be traced in the available literature. The objective of the present work was to investigate the lipoidal matter from seeds and to isolate and identify the major sterols and triterpenes in the petroleum ether extract.
Materials and methods
The petroleum ether extract of seeds was prepared. The extract was subjected to column chromatography using silica gel (type 60-230 mesh) as an adsorbent. The unsaponifiable matter and fatty acid methyl esters were analyzed by GC/MS.
Results and conclusion
GC/MS analysis of the unsaponifiable matter from the petroleum ether extract of seeds revealed the identification of 70.04% of unoxygenated compounds and 23.34% oxygenated compounds. Hydroxylated compounds, ketones, and steroidal compounds represent 23.24, 0.20, and 3.19%, respectively. 1-Heptene (66.47%) was the major compound in the USM, followed by butylated hydroxy toluene (21.07%). GC/MS analysis of fatty acid methyl esters showed that nine fatty acid methyl ester derivatives could be identified, representing 95.84% of the total composition. The major fatty acid was 9,12-octadecadienoic acid (linoleic acid) (33.8%). Saturated fatty acids represent 36.93% of the total fatty acid content, whereas monounsaturated and diunsaturated fatty acids represent 25.11 and 33.8% of the total fatty acid content, respectively. Column chromatography of the petroleum ether extract led to the isolation of two compounds [α–amyrin and 5,24(28)-cholestadien-24-methylen-3β-ol].

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