Inhibition of human pathogenic bacteria by cultivated in Jazan (Kingdom of Saudi Arabia) and study of synergy to amoxicillin

Background
The spread of multidrug-resistant strains of bacteria to most antibiotics has led to intensive searches for effective strategies to overcome bacterial infections.
Aim

could be a potentially useful agent for many therapeutic applications, especially antimicrobial.
Settings and design
The leaves of were collected in October 2014 from Jazan, located in the southern region of the Kingdom of Saudi Arabia.
Materials and methods
Different organic solvents were used to extract antimicrobial substances in the plant leaves. The antibacterial activity of each leaf extract was determined and compared with some antibiotics. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of both plant extracts and amoxicillin were investigated against spp. An evaluation of the synergistic effects of amoxicillin and plant extract was carried out.
Results
The results showed that an ethanol extract is the best organic solvent for extracting the antimicrobial substance from . In addition, the antibacterial potential of an ethanol extract is comparable to that of some commercial antibiotics. MIC and MBC of the plant extract were 320 and 620 μg/ml, respectively, whereas MIC and MBC of amoxicillin were 25 and 50 μg/ml, respectively. The fractional inhibitory concentration of the plant extract and amoxicillin was determined to be 0.125 and 0.25, respectively. Therefore, ethanolic plant extract can be considered a good synergistic factor to amoxicillin, yielding a fractional inhibitory concentration index 0.375 ≤ 0.5 of the combination.
Conclusion

leaves may serve as a natural alternative to antibiotics. Moreover, can boost the inhibitory effect of amoxicillin, leading to a reduction of administration dose as well as minimizing the side effects of antibiotics.