Possible role of a compound identified from  plant in controlling blood glucose in experimental animals

Kifayatullah, Muhammad; Mustapha, Mohammad S.; Sarker, Mohammad M.R.; Kaleemullah,; Amin, Muhammad; Ali, Fawad

doi:10.4103/1687-4315.205824

Possible role of a compound identified from plant in controlling blood glucose in experimental animals

Authors

10.4103/1687-4315.205824

Abstract

Objective
The aim of the present research work was to determine the possible mechanism of an active fraction identified from plant in controlling blood glucose in experimental animals.
Materials and methods
The effect of an active fraction from on intestinal absorption of glucose at a dose of 50 mg/kg (body weight) was investigated using an in-vivo method. The identification of the compound was carried out with gas chromatography mass spectrometry followed by H nuclear magnetic resonance. The active fraction and standard drug were given daily for 7 days. The animal groups were anesthetized through intraperitoneally injection with sodium pental at a dose of 40 mg/kg (body weight) after they were made to fast overnight to evaluate the effect of the active fraction on the absorption of glucose in comparison with the diabetic group.
Results and discussions
A single dose of 50 mg/kg (body weight) of the active fraction and standard drug acarbose produced significant (<0.001) attenuations in the intestinal absorption of glucose as compared with the diabetic group. The amount of glucose absorbed was 94.05±0.9 mg/g tissue (weight) in the diabetic group, whereas that absorbed in the active fraction-treated group was 55.80±1.2 mg/g tissue (weight). However, the animals treated with standard drug acarbose showed a significant reduction in glucose absorption rate at 38.21±2.5 mg/g tissue (weight). The inhibition of glucose absorption by standard acarbose and active fraction was 59.37 and 40.66%, respectively, as compared with the diabetic control group. The gas chromatography mass spectrometry analysis indicated a single compound propanoic acid in plant sample. The molecular weight was 234, H nuclear magnetic resonance was 452 MHz, %area was 3.57, retention time was 2.990, and total area was 1 015 462. The molecular formula was CHOSi at / 73 (high resolution). The presence of this compound in might be responsible for the inhibition of glucose absorption by blocking Na, K ATPases activity.
Conclusion
The present experiment confirmed reduced glucose absorption rate and the antidiabetic activity is due to this possible mechanism.

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