GC-MS analysis and  hypocholesterolemic, anti-rotavirus, anti-human colon carcinoma activities of the crude extract of a Japanese  spp

Elkhateeb, Waill A.; Daba, Ghoson M.; Sheir, Donia; El-Dein, Asmaa Negm; Fayad, Walid; Elmahdy, ELmahdy M.; Shaheen, Mohamed N.F.; Thomas, Paul W.; Wen, Ting-Chi

doi:10.4103/epj.epj_50_18

GC-MS analysis and hypocholesterolemic, anti-rotavirus, anti-human colon carcinoma activities of the crude extract of a Japanese spp

Authors

Abstract

Background and objective
Medicinal mushrooms are mines of various biologically active compounds. Therefore, chemical analysis and evaluation of some biological activities of the Japanese originated mushroom spp. were conducted.
Materials and methods
Extraction of the fruiting bodies of spp. was accomplished using 80% methanol. This extract was investigated for its cholesterol-lowering activity, anti-rotavirus effect, and anti-human colon cancer influence. Moreover, a gas chromatography–mass spectrometry analysis for this extract was performed.
Results and conclusion
The gas chromatography–mass spectrometry analysis resulted in the detection of 39 compounds, which were generally saturated and unsaturated fatty acids, and alkenes. The crude extract exhibited a promising cholesterol-lowering activity (100±0%) after 96 h of incubation at room temperature. The same crude extract showed a moderate anti-rotavirus SA-11 strain effect with a therapeutic index of 9.3. Moreover, spp. extract displayed a strong activity toward HCT116 human colon carcinoma cell line, resulting in a cytotoxicity of 84.03±0.93% on HCT116 cell line monolayers. spp. crude extract represents a promising source of biologically active compounds that could by further investigations represent support and/or alternative to the currently used drugs.

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