Investigation of lipoidal matter of  seeds and its cytotoxicity potential against three human cancer cell lines

Bata, Shaymaa M.; Shams, Khaled A.; Khalil, Ali K.; Hassan, Rasmia A.; Saleh, Ibrahim A.; Hamed, Ahmed R.; Abdel-Azim, Nahla S.; Hammouda, Faiza M.

doi:10.4103/epj.epj_53_19

Investigation of lipoidal matter of seeds and its cytotoxicity potential against three human cancer cell lines

Authors

Abstract

Background and objectives
Quinoa ( Willd) is a plant species that belongs to Chenopodiaceae family. It has a high nutritional value as it is rich in proteins, lipids, and fiber, and has an extraordinary balance of essential amino acids, vitamins, and minerals, in addition to a high number of health-beneficial phytochemicals, including saponins and phytosterols. The present study aims to investigate the lipoidal components of and assessment of lipoidal components and different fractions (methanol, chloroform, ethylacetate, and butanol) cytotoxicity potential against three human cancer cell lines using MTT reduction assay.
Materials and methods
The seeds of were extracted by different solvents and the extracts prepared were tested for their lipiodial constituents and cytotoxic activities in three cancer cell lines.
Results and conclusion
The results obtained from Gas Liquid Chromatography (GLC) analysis of nonsaponifiable fraction of seeds indicated that it consists mainly of a mixture of a series of -alkanes and sterols (79.96 and 7.91%. respectively). The oil was also found to be rich in linoleic acid (52.7%) and oleic acid (25.33%). The investigation of the cytotoxic potential of the plant’s low polarity fractions, including CHCl, and nonsaponifiable fraction against the studied cell lines revealed the sensitivity of A549 human lung cancer cells. Further studies are needed to uncover the mechanism and chemical constituents of the obtained activity of .

Keywords