Insights into the in-vitro hypocholesterolemic, antioxidant, antirotavirus, and anticolon cancer activities of the methanolic extracts of a Japanese lichen, , and a Japanese mushroom,

Elkhateeb, Waill A.; Daba, Ghoson M.; El-Dein, Asmaa N.; Sheir, Donia H.; Fayad, Walid; Shaheen, Mohamed N.; Elmahdy, ELmahdy M.; Wen, Ting-Chi

doi:10.4103/epj.epj_56_19

Insights into the in-vitro hypocholesterolemic, antioxidant, antirotavirus, and anticolon cancer activities of the methanolic extracts of a Japanese lichen, , and a Japanese mushroom,

Authors

Abstract

Background and objective
Medicinal mushrooms, as well as lichens are potential sources of bioactive compounds against many lethal diseases. Therefore, in-vitro evaluations of the biological activities of the 80% methanolic extracts of two Japanese originated samples, namely, the mushroom , and the lichen have been performed.
Materials and methods
Extraction of and was conducted using 80% methanol. The resulted extracts were examined for their in-vitro hypocholesterolemic and antirotavirus activities, as well as antihuman colon cancer action.
Results and conclusion
It was revealed that both extracts have potent hypocholesterolemic effects. in concentration of 24 mg/ml could successfully reduce the cholesterol concentration to 100±0% after 96 h of incubation at room temperature, whereas reaching this degree of reduction in cholesterol concentration required using 32 mg/ml of extract. On the contrary, the extract of at concentration of 1.0 mg/ml exhibited a stronger DPPH scavenging activity (99.5±0.166%), in comparison with 66.24±0.43% in case of using the same concentration of the extract. extract showed antirotavirus in a therapeutic index of 11, whereas extract recorded 3.4 only. The antihuman colon cancer study elucidated that both extracts have a moderate activity toward HCT116 human colon carcinoma. extract achieved a cytotoxicity of 57.90±4.4%, whereas the extract of displayed only 43.10±0.9%. Studies on and introduce them as promising sources of biologically potent compounds that can be candidates for use in treatment of many diseases after further studies.

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