and leaf fractions ameliorate the adverse effects of indomethacin in ovary and serum of treated rats

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Abstract

Background and objective

plants potentially contain bioactive principles against reproductive toxicants and oxidative stress. Thus, the ameliorative action of methanol and ethyl acetate fractions of and leaves on cyclooxygenase-2 (COX-2) and oxidative-stressed states in indomethacin-induced rat tissues have been performed.
Materials and methods
The dried-leaf extract of and was subjected to liquid–liquid fractionation to obtain the ethyl acetate and methanol fractions. The ethyl acetate and methanol fractions were respectively administered orally to rats (200 mg/kg), 30 min, and 10 h after subcutaneous injection with indomethacin (5 mg/kg) for 4 days. The postadministration of and fractions was used to determine their effect on ovarian and serum COX-2 concentration, ovarian malondialdehyde, and ovarian nitric oxide concentrations.
Results and conclusion

and fractions significantly (<0.05) increased the concentration of COX-2 in ovaries and serum compared with the group treated with indomethacin only. Malondialdehyde and nitric oxide concentrations were significantly (<0.05) decreased in all the animal groups posttreated with plant fractions compared with indomethacin only. Histological assessment of the ovary showed proliferating ovarian follicles and mature Graafian follicles in the groups treated with the plant fractions, while the indomethacin-only group showed scanty primary follicles. These results showed that and leaf fractions mediated their protective effect on the ovaries and serum through the regulated COX-2 action and inhibited indomethacin-induced oxidative stress.

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Egyptian Pharmaceutical Journal
Volume 21, Issue 2
April 2022
Pages 109-116

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