A new terpenoid from  L. leaves and potential aptitude of different leaf extracts as anti-inflammatory agents

Ghanem, Mona T.M.; Tawfik, Wafaa A.; Mahdy, El-Sayed M.; Abdelgawad, Mohamed E.; Mohamed, Tarik A.; Hamed, Ahmed R.; Ohta, Shingi; Hegazy, Mohamed Elamir F.; Abdel-Azim, Nahla S.; Shahat, Abdelaaty A.; El-Missiry, Moustafa M.

doi:10.4103/epj.epj_131_22

A new terpenoid from L. leaves and potential aptitude of different leaf extracts as anti-inflammatory agents

Authors

Abstract

Background
The olive tree ( L.) has played a significant role in medical history, being used to relieve a wide range of illnesses in traditional medicine. The value of olive leaves came from their medicinal and nutritional properties. In recent times, it has been proven that there is a potential role of leaves in cancer prevention and inflammatory illnesses.
Objective
The purpose of this study was to look at the chemical makeup of methylene chloride fraction and also to evaluate the chemopreventive properties and the anti-inflammatory activity of different olive leave fractions using hepa1c1c7 cells and RAW264.7 macrophages activated by lipopolysaccharides, respectively.
Materials and methods
Air-dried powdered olive leaves were defatted with hexane, and the marc was then soaked in 80% methanol and successively extracted with CHCl, EtOAc, and n-BuOH. Phytochemical investigation of CHCl fraction was done. The chemopreventive effect was determined using a hepa1c1c7 human carcinoma cell line, and the anti-inflammatory was carried out using RAW264.7 macrophages.
Results and conclusion
One novel compound, identified and given the name oleuropeinone (
1
), was obtained together with two known compounds, blumenol A (
2
) and megaritolactonol (
3
). Spectroscopic data such as H and C NMR were used to identify their chemical structures. The CHCl fraction is the most effective fraction to inhibit inflammatory markers inducible nitric oxide synthase and COX-2 protein expression in RAW264.7 macrophages. Moreover, it has a potent effect in inducing the chemopreventive marker NQO1 protein expression.

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