Effects of  extract on 6-hydroxydopamine neurotoxicity in differentiated SH-SY5Y cells

Bitmez, Baris; Gultekin, Seda K.; Albayrak, Irem G.; Deveci, Yigit; Sicak, Yusuf; Akalin, Emine; Pirhan, Adami F.; Gurer, Ulas; Arslan, Belkis A.

doi:10.4103/epj.epj_180_22

Effects of extract on 6-hydroxydopamine neurotoxicity in differentiated SH-SY5Y cells

Authors

Abstract

Background and objective
Parkinson’s disease (PD) is the second most common neurodegenerative disease after Alzheimer’s disease. In our study, PD model was created as a result of exposure to 6-hydroxydopamine (6-OHDA) in SH-SY5Y cells, which is a human neuroblastoma cell line. The protective effect of on PD was investigated.
Materials and methods
Phytochemical analysis of extract was performed. Then, SH-SY5Y cells were differentiated using retinoic acid and then administered 6-OHDA neurotoxin. To determine the protective effects of extract, we investigated the changes in the mRNA expression level of caspase-3, total oxidant status, and antioxidant levels in differentiated SH-SY5Y.
Results and conclusion
According to our results, extract contains glycosides, tannins, flavonoids, and carbohydrates as the major secondary metabolites. extract significantly reduced caspase-3 gene expression against 6-OHDA toxicity in differentiated SH-SY5Y cells. It was found that total oxidant status level increased significantly in the 6-OHDA experimental group compared with the control and experimental groups. It was found that extract has an inhibitory effect on caspase-3 gene expression, which plays an important role in apoptosis. Therfore extract has been shown to have a therapeutic potential against 6-OHDA toxicity.

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